Defect-induced activity enhancement of enzyme-encapsulated metal-organic frameworks revealed in microfluidic gradient mixing synthesis

Chong Hu; Yunxiu Bai; Miao Hou; Yisu Wang; Licheng Wang; Xun Cao; Chiu-Wing Chan; Han Sun; Wanbo Li; Jun Ge; Kangning Ren

doi:10.1126/sciadv.aax5785

Research ArticleBIOCHEMISTRY

Defect-induced activity enhancement of enzyme-encapsulated metal-organic frameworks revealed in microfluidic gradient mixing synthesis

View ORCID ProfileChong Hu ¹,³,*,
View ORCID ProfileYunxiu Bai²,*,
Miao Hou²,
View ORCID ProfileYisu Wang¹,
Licheng Wang²,
Xun Cao²,
View ORCID ProfileChiu-Wing Chan¹,
View ORCID ProfileHan Sun¹,
View ORCID ProfileWanbo Li¹,
View ORCID ProfileJun Ge ²,⁴,† and
View ORCID ProfileKangning Ren ¹,⁵,⁶,†

¹Department of Chemistry, Hong Kong Baptist University, Hong Kong, P. R. China.
²Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, P. R. China.
³School of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, P. R. China.
⁴Biopharmaceutical and Health Engineering Division, Tsinghua Shenzhen International Graduate School, Shenzhen, P. R. China.
⁵State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong, P. R. China.
⁶HKBU Institute of Research and Continuing Education, Shenzhen, P. R. China.

↵†Corresponding author. Email: kangningren{at}hkbu.edu.hk (K.R.); junge{at}mail.tsinghua.edu.cn (J.G.)

↵* These authors contributed equally to this work.

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Science Advances 29 Jan 2020:
Vol. 6, no. 5, eaax5785
DOI: 10.1126/sciadv.aax5785

Chong Hu

1Department of Chemistry, Hong Kong Baptist University, Hong Kong, P. R. China.

3School of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, P. R. China.

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Yunxiu Bai

2Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, P. R. China.

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Miao Hou

2Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, P. R. China.

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Yisu Wang

1Department of Chemistry, Hong Kong Baptist University, Hong Kong, P. R. China.

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Licheng Wang

2Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, P. R. China.

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Xun Cao

2Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, P. R. China.

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Chiu-Wing Chan

1Department of Chemistry, Hong Kong Baptist University, Hong Kong, P. R. China.

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Han Sun

1Department of Chemistry, Hong Kong Baptist University, Hong Kong, P. R. China.

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Wanbo Li

1Department of Chemistry, Hong Kong Baptist University, Hong Kong, P. R. China.

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Jun Ge

2Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, P. R. China.

4Biopharmaceutical and Health Engineering Division, Tsinghua Shenzhen International Graduate School, Shenzhen, P. R. China.

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For correspondence: kangningren@hkbu.edu.hk junge@mail.tsinghua.edu.cn

Kangning Ren

1Department of Chemistry, Hong Kong Baptist University, Hong Kong, P. R. China.

5State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong, P. R. China.

6HKBU Institute of Research and Continuing Education, Shenzhen, P. R. China.

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For correspondence: kangningren@hkbu.edu.hk junge@mail.tsinghua.edu.cn

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Defect-induced activity enhancement of enzyme-encapsulated metal-organic frameworks revealed in microfluidic gradient mixing synthesis

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