Research ArticleGENETICS

Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9–mediated genome editing events

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Science Advances  12 Feb 2020:
Vol. 6, no. 7, eaax2941
DOI: 10.1126/sciadv.aax2941

Article Information

vol. 6 no. 7

Online ISSN: 
History: 
  • Received for publication March 11, 2019
  • Accepted for publication November 25, 2019
  • .

Author Information

  1. Boris V. Skryabin1,*,
  2. Delf-Magnus Kummerfeld1,
  3. Leonid Gubar1,
  4. Birte Seeger1,
  5. Helena Kaiser1,
  6. Anja Stegemann1,
  7. Johannes Roth2,
  8. Sven G. Meuth3,
  9. Hermann Pavenstädt4,
  10. Joanna Sherwood5,
  11. Thomas Pap5,
  12. Roland Wedlich-Söldner6,
  13. Cord Sunderkötter7,
  14. Yuri B. Schwartz8,
  15. Juergen Brosius9,10 and
  16. Timofey S. Rozhdestvensky1,*
  1. 1Medical Faculty, Core Facility Transgenic Animal and Genetic Engineering Models (TRAM), University of Muenster, Muenster, Germany.
  2. 2Institute of Immunology, University Hospital Muenster, Muenster, Germany.
  3. 3Clinic of Neurology with Institute of Translational Neurology, University Hospital Muenster, Muenster, Germany.
  4. 4Internal Medicine D, University Hospital Muenster, Muenster, Germany.
  5. 5Institute of Experimental Musculoskeletal Medicine (IMM), University Hospital Muenster, Muenster, Germany.
  6. 6Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.
  7. 7Department of Dermatology and Venereology, University Hospital Halle, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
  8. 8Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden.
  9. 9Institute of Experimental Pathology (ZMBE), University of Muenster, Muenster, Germany.
  10. 10Institutes for Systems Genetics, West China Hospital, Sichuan University, Chengdu 610041, China.
  1. *Corresponding author. Email: skryabi{at}uni-muenster.de (B.V.S.); rozhdest{at}uni-muenster.de (T.S.R.)

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