Science Advances

Supplementary Materials

This PDF file includes:

  • CaCas9 protein sequence
  • Fig. S1. Recyclable Solo system vector pV1200 permits serial mutagenesis.
  • Fig. S2. Candida CRISPR-Duet system requires Cas9, sgRNA expression, and a mutagenic repair template.
  • Fig. S3. Candida CRISPR-Solo system requires a mutagenic repair template, but does not require selection for system components.
  • Fig. S4. Candida CRISPR permits the isolation of homozygous mutants at multiple loci, including MtlA1, Mtla2, TPK2, and DCR1.
  • Fig. S5. Mutation of CDR1 and CDR2 creates pleiotropic drug sensitivity.
  • Fig. S6. Mutation of SNF1 in Candida.
  • Table S1. Plasmids used in this study.

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Other Supplementary Material for this manuscript includes the following:

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