Science Advances
Supplementary Materials
This PDF file includes:
- General Methods
- Fig. S1. Dose-dependent activation of SEAP signaling by analogs in HEK-Blue hTLR2 cells after 24 hours.
- Fig. S2. The MTT cell viability of HEK-Blue hTLR2 cells after 24 hours of incubation with CU-T12-9 and antibodies.
- Fig. S3. NO activation of CU-T12-9 can be suppressed by a TLR1/2 antagonist, but not by a TLR4 antagonist.
- Fig. S4. Anisotropy assays for TLR1, TLR2, or TLR1/2 protein binding to rhodamine-labeled Pam3CSK4 (Rho-Pam3).
- Fig. S5. Binding of CU-T12-9 to TLR1 by MST.
- Fig. S6. Binding of CU-T12-9 to TLR2 by MST.
- Fig. S7. TLR1 and TLR2 oligomeric states as seen by SEC-LS.
- Fig. S8. Concentration-dependent 1H NMR experiments.
- Fig. S9. HEK-Blue hTLR2 and Raw 264.7 cell viability upon CU-T12-9 treatment.
- Fig. S10. Protein expression and characterization.
- Table S1. SAR studies of the GA analogs in activation of SEAP signaling in HEK-Blue hTLR2 cells.
- Scheme S1. General synthesis of TLR1/2 agonist GA.
- Synthesis and experimental data.
- References (52–54)
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