Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Plasmids built for reconstruction of the circadian oscillator in E. coli.
  • Fig. S2. Additional data and quantifications of KaiC phosphorylation in E. coli expressing KaiABC.
  • Fig. S3. Circadian phosphorylation of KaiC over time requires KaiA and KaiB.
  • Fig. S4. Plasmids built for the synthetic oscillator utilizing a modified bacterial two-hybrid system.
  • Fig. S5. KaiC and SasA phosphorylation states affect reporter output.
  • Table S1. Raw data for Fig. 2D.
  • Table S2. Bacterial strains and plasmids.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Circadian oscillations visualized in single E. coli cells using a microfluidic device.

Files in this Data Supplement: