Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Ankyrin-G patterns into micrometer-scale domains in mouse kidney epithelial lateral membranes.
  • Fig. S2. Latrunculin A and nocodazole treatments effectively depolymerize the MDCK microtubule and actin cytoskeleton, respectively.
  • Fig. S3. Stabilization of microtubules or actin does not affect dynamics of ankyrin-G microdomains or lateral membrane coverage.
  • Fig. S4. Microtubules colocalize with ankyrin-G microdomains at discrete sites and are required for lateral membrane biogenesis.
  • Legends for movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Live imaging of ankyrin-G–GFP on the lateral membrane of control MDCK cells.
  • Movie S2 (.avi format). Live imaging of ankyrin-G–GFP on the lateral membrane of paraformaldehyde-fixed MDCK cells.
  • Movie S3 (.avi format). Live imaging of βII-spectrin–GFP on the lateral membrane of MDCK cells treated with latrunculin A.
  • Movie S4 (.avi format). Live imaging of ankyrin-G–GFP on the lateral membrane of MDCK cells treated with latrunculin A.
  • Movie S5 (.avi format). Live imaging of ankyrin-G–GFP on the lateral membrane of MDCK cells treated with nocodazole.

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