Science Advances

Supplementary Materials

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  • Fig. S1. Schematic of the different constructs of the human β-cardiac sS1 used in our experiments and relative purity of the wild type and R403Q protein preparations.
  • Fig. S2. Representative experimental traces of wild-type and R403Q sS1 unloaded motility.
  • Fig. S3. Single-molecule stroke size measurements of wild type and R403Q human β-cardiac sS1.
  • Table S1. Rate constants and equilibrium constants for the various kinetic steps of wild type and R403Q human β-cardiac sS1.
  • Reaction schemes
  • Scheme 1. Kinetic schemes for interaction of myosin only with nucleotide.
  • Scheme 2. Kinetic schemes for interaction of myosin with actin and nucleotide.
  • Scheme 3. A combined equilibrium scheme for myosin, actin, and ADP binding to each other.
  • Reference (70)

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