Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Modification of an open-source 3D printer for FRESH printing.
  • Fig. S2. Preparation of the gelatin slurry support bath.
  • Fig. S3. Examples of 3D printed bifurcated tubes using alginate, fibrin, and collagen.
  • Fig. S4. 3D printed sheets of cells and ECM.
  • Fig. S5. Mechanical characterization of cast and 3D printed alginate dog bones using uniaxial tensile testing.
  • Fig. S6. A comparison of the 3D model and 3D printed arterial tree to assess print fidelity.
  • Fig. S7. A 3D printed perfusion fixture for the right coronary arterial tree.
  • Fig. S8. Generation of a 3D model of the embryonic heart from confocal microscopy.
  • Fig. S9. A comparison of the 3D model and 3D printed embryonic heart to assess print fidelity.
  • Fig. S10. A comparison of the 3D model and 3D printed brain.
  • Legends for movies S1 to S10

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Time lapse of FRESH printing and heated release of the "CMU" logo.
  • Movie S2 (.mp4 format). FRESH printing using the dual syringe pump extruders.
  • Movie S3 (.mp4 format). Out-of-plane FRESH printing of a helix.
  • Movie S4 (.mp4 format). Uniaxial strain of a FRESH printed femur model showing elastic recovery.
  • Movie S5 (.mp4 format). Strain to failure of a FRESH printed femur.
  • Movie S6 (.mp4 format). FRESH printing of soft collagen type I constructs.
  • Movie S7 (.mp4 format). Time-lapse video of a coronary arterial tree being FRESH printed.
  • Movie S8 (.mp4 format). Perfusion of a FRESH printed coronary arterial tree.
  • Movie S9 (.mp4 format). Visualization of the 3D structure of a FRESH printed brain model.
  • Movie S10 (.mp4 format). Modification of a sub-$400 3D printer for FRESH printing.

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