Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. UPS that developed in a Neu1+/−/Arf−/− mouse expresses epithelial and mesenchymal markers and TGFβ.
  • Fig. S2. Excessive lysosomal exocytosis in RMS cells is caused by low NEU1 and high LAMP1 levels.
  • Fig. S3. Genes whose expression is negatively correlated with that of NEU1 expression in a sarcoma data set (GSE21050).
  • Fig. S4. Showing of entire immunoblots corresponding to panels in Fig. 5 (E and F).
  • Fig. S5. Immunoprecipitation of Myosin-11 and CD63.
  • Fig. S6. NEU1 expression levels in tumor cells and the surrounding microenvironment determine their invasiveness.
  • Fig. S7. Increased lysosomal exocytosis confers chemoresistance to RMS cells.
  • Table S1. Type of tumors developed in Neu1+/+/Arf−/− and Neu1+/−/Arf−/− mice.
  • Table S2. Immunohistochemical markers used for diagnosis of sarcomas.
  • Table S3. Metastasis status in the sarcoma data set (GSE21050).
  • Legends for movies S1 to S9

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). In RH41 cells, lysosomes localize in the perinuclear region.
  • Movie S2 (.mov format). In RH30 cells, lysosomes distribute to the cell periphery.
  • Movie S3 (.mov format). Live TIRF imaging of LysoTracker-labeled lysosomes in RH41 as a measure of tethering to or docked at the PM.
  • Movie S4 (.mov format). Live TIRF imaging of LysoTracker-labeled lysosomes in RH30 as a measure of tethering to or docked at the PM.
  • Movie S5 (.mov format). Doxorubicin exposure induces lysosome aggregation and subsequent apoptosis of RH41 cells.
  • Movie S6 (.mov format). RH30 cells maintain lysosomal trafficking and are resistant to doxorubicin exposure.
  • Movie S7 (.mov format). In RH41 cells, doxorubicin concentrates primarily in the nucleus.
  • Movie S8 (.mov format). In RH30 cells, a fraction of doxorubicin remains lysosomal.
  • Movie S9 (.mov format). RH30 cells cotreated with doxorubicin and verapamil cluster lysosomes in the perinuclear region and undergo apoptosis.

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