Science Advances

Supplementary Materials

This PDF file includes:

  • Table S1. Expected and actual PCR sizes (kb) of various strains.
  • Legends for tables S2 to S4
  • Table S5. Primers used in this study.
  • Table S6. HPLC analysis of fermentation products.
  • Fig. S1. Modular architectures of the putative secondary scaffoldin Cthe_0736 (ScaE) and the primary scaffoldin CipA, as well their three purified recombinant proteins expressed in E. coli.
  • Fig. S2. Cohesins of the putative secondary scaffoldin ScaE binding to type II dockerin of CipA revealed by native PAGE.
  • Fig. S3. Identification of correct gene deletion in various mutants by PCR.
  • Fig. S4. Identification of scaE deletion by PCR.
  • Fig. S5. Correlations between biological and technical replicates analyzed by microarray.
  • Fig. S6. Identification of CipA protein in the exoproteomes of mutants by SDS-PAGE.
  • Fig. S7. Exoproteome protein abundances cluster into two distinct groups: samples with an intact CipA (DS3 and CTN5) and those without (DS11, CTN4, and CTN7).
  • Fig. S8. Sample-to-sample correlation at the protein level (actual values).
  • Fig. S9. PCA depicts two major sample groups: those with an intact CipA (DS3 and CTN5) and those with CipA deleted (DS11, CTN4, and CTN7).

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Data set of transcriptomic profiling results for C. thermocellum DSM1313 scaffoldin deletion strains detected by microarray.
  • Table S3 (Microsoft Word format). Table of the ranked abundance for selected cellulosome and cellulase genes.
  • Table S4 (Microsoft Excel format). Supernatant proteomic profiling results for C. thermocellum DSM1313 scaffoldin deletion strains measured by LC-MS/MS.

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