Science Advances

Supplementary Materials

This PDF file includes:

  • Fig S1. Monotypica cultures of human trophoblast cells lines are not compatible with culturing in the RWV bioreactor.
  • Fig S2. Coculturing of JEG-3 cells with human microvascular endothelial cells promotes their attachment to Cytodex beads in the RWV bioreactor.
  • Fig S3. Human microvascular cells are removed from Cytodex beads by JEG-3 cells.
  • Fig S4. Levels of pregnancy hormones increases during culturing of JEG-3 cells in 3D.
  • Fig S5. JEG-3 cells cultured in 3D express high levels of syncytin, form brush borders, and can be transfected with siRNAs.
  • Fig S6. GSEA plots of genes with higher or lower abundance in JEG-3 cells cultured in 2D or 3D or in primary human trophoblasts.
  • Table S1. Thirteen “core” genes identified using GSEA gene clustering as being up-regulated in both 3D JEG-3 and PHT cells, while being of low abundance in both 2D JEG-3 cells and 3D HBMECs.
  • Table S2. Spreadsheet of gene expression profiles from RNASeq in 2D and 3D cultures of JEG-3 cells, PHT cells, and 3D cultures of HBMECs.
  • Legends for date set S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Data set S1 (Microsoft Excel format). Spreadsheet from RNASeq studies of 2D and 3D cultures of JEG-3 cells, PHT cells, and 3D cultures of HBMECs. Shown are gene symbols, normalized expression values, and RPKM values from each condition.
  • Data set S2 (Microsoft Excel format). Spreadsheet from differential expression analyses using DESeq2 of 2D and 3D cultures of JEG-3 cells.
  • Data set S3 (Microsoft Excel format). Spreadsheet from differential expression analyses using DESeq2 of 2D and 3D cultures of HBMECs.
  • Data set S4 (Microsoft Excel format). Spreadsheet from differential expression analyses using DESeq2 of 2D cultures of JEG-3 cells and PHT cells.

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