Science Advances

Supplementary Materials

This PDF file includes:

  • Shape analysis of faceted vesicles
  • His-anillin binding to the actin network and to the membrane
  • Estimates of binding affinities
  • Membrane/network bound anillin
  • table S1. Calculated bound anilin concentration under different conditions.
  • fig. S1. Actin network in vesicles in the absence of Ni-NTA lipids.
  • fig. S2. Different contributions of anillin.
  • fig. S3. Controls of membrane functionalization and encapsulation.
  • fig. S4. Determination of the ratio of cortical anillin.
  • fig. S5. Size distribution of the big stable blebs.
  • fig. S6. Circularity of faceted vesicles containing 0.5 μM myosin.
  • fig. S7. Phase diagram with fluorescent images.
  • Legend for movie S1
  • References (38–40)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi format). Fluctuations of the lipid membrane before and after complete actomyosin contraction.

Files in this Data Supplement: