Science Advances

This PDF file includes:

• Shape analysis of faceted vesicles
  
• His-anillin binding to the actin network and to the membrane
  
• Estimates of binding affinities
  
• Membrane/network bound anillin
  
• table S1. Calculated bound anilin concentration under different conditions.
  
• fig. S1. Actin network in vesicles in the absence of Ni-NTA lipids.
  
• fig. S2. Different contributions of anillin.
  
• fig. S3. Controls of membrane functionalization and encapsulation.
  
• fig. S4. Determination of the ratio of cortical anillin.
  
• fig. S5. Size distribution of the big stable blebs.
  
• fig. S6. Circularity of faceted vesicles containing 0.5 μM myosin.
  
• fig. S7. Phase diagram with fluorescent images.
  
• Legend for movie S1
  
• References (38–40)

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Other Supplementary Material for this manuscript includes the following:

• movie S1 (.avi format). Fluctuations of the lipid membrane before and after complete actomyosin contraction.