Science Advances

Supplementary Materials

This PDF file includes:

  • section SI. Supplementary materials, methods, and figures
  • section SII. Determination of the formin-induced, barbed-end polymerization rate
  • section SIII. Single-filament computations to determine effective molecular rates
  • section SIV. Determination of filament length distribution in the subpopulations
  • section SV. Filament severing
  • section SVI. F-actin abundances in the actin cortex
  • fig. S1. Experimental setup.
  • fig. S2. Single-step photobleaching control by a temporal evolution of the molecule fluorescence intensity.
  • fig. S3. (A and B) Lifetime distribution with average lifetime ωoff, F = 0:12 ± 0:1
    of (A) Diaph1 (N = 1000 molecules) and of (B) CA-Diaph1 (N = 3000 molecules).
  • fig. S4. Actin filament fractions depend on the cortical nucleator concentrations.
  • fig. S5. Fraction Φ of immobile molecules as a function of the average filament length λ according to eq. S5 with p(ℓ) = exp(−ℓ/λ)/λ.
  • fig. S6. Effects on molecule mobility.
  • fig. S7. Illustration of monomer lifetimes during continuous filament growth.
  • fig. S8. Simulated FRAP curves for scenarios including filament severing.
  • table S1. Comparison between model parameters used in the finite cortex simulations and literature values.
  • table S2. Parameter values used in the simulations of FRAP experiments.
  • table S3. Parameter values for the two scenarios of filament severing.
  • References (71–73)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi format). Fluorescence imaging of cortical Diaph1-GFP in a HeLa cell corresponding to Fig. 1A.
  • movie S2 (.avi format). Fluorescence imaging of cortical actin-GFP in an M2 cell.
  • movie S3 (.avi format). Detail of fluorescence imaging of cortical Diaph1-GFP in a HeLa cell corresponding to Fig. 1B.
  • movie S4 (.avi format). Cortex simulation of a FRAP/FLAP experiment in a HeLa cell.

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