Science Advances

Supplementary Materials

This PDF file includes:

  • Bicinonic Acid Assay (BCA)
  • Surface tension experimental details
  • Surface tensions for control samples at RT
  • XPS experimental details
  • fig. S1. Fitting results for SFG spectra.
  • fig. S2. SFG spectra recorded in the amide I region for P. syringae at the air-water
    interface at RT and 5°C.
  • fig. S3. Time traces of surface tension changes recorded while the temperature
    was changed in the trough for D2O and Snomax at the air-water interface.
  • fig. S4. Surface tension changes relative to the tension at 22°C for pure D2O and
    the Snomax sample for sample temperatures studied with SFG.
  • fig. S5. XPS spectra of P. syringae films adsorbed at the water-air interface at 22°
    and 5°C and transferred to gold-coated silicon chips following the Langmuir-Schaefer (54) method.
  • fig. S6. XPS survey spectrum of the Snomax sample.
  • fig. S7. Droplet freeze assay for the P. syringae sample used in this study.
  • fig. S8. Illustration of the simulation box.
  • fig. S9. Calculated SFG intensity | χ xxz2(ω)|2) for the O–D stretch chromophores
    within 15 Å of the center of mass of the IN dimer in the z direction.
  • fig. S10. Snapshot of the MD simulation near the Thr-rich region (Fig. 3, region
    2, main text) of an IN site.
  • fig. S11. Time-resolved difference sum frequency spectra for control substances.
  • fig. S12. Energy transfer processes at the water-AFP interface.
  • table S1. Elemental compositions (atom %) determined by XPS of protein monolayers deposited onto gold-coated silicon chips with the Langmuir-Schaefer technique.
  • table S2. Time scales (in femtoseconds) extracted from the coupled differential equation fits (only down was allowed to float; the others were fixed).
  • References (55–68)

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