Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • fig. S1. DNA construct design, ESC colony morphology, DNA integration validation, and pluripotency characterization.
  • fig. S2. Electrophysiological characterization of the ChR-HBG3-MN.
  • fig. S3. Optogenetic activation of NMJs in adherent cocultures.
  • fig. S4. Modes of tissue ablation.
  • fig. S5. Photosensitization of the C2C12 myoblasts.
  • fig. S6. Influence of the hydrogel on the force measured by the pillar.
  • fig. S7. Motor neurite outgrowth in the microfluidic device.
  • fig. S8. Opposite gradients as a mean to provide cell-specific medium.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi format). Muscle contraction upon optical stimulation of ChR-HBG3-MN in adherent cultures.
  • movie S2 (.avi format). ECM compaction and muscle bundle formation over the first 96 hours of the experiment.
  • movie S3 (.wmv format). 3D neurite outgrowth in the gel region bridging the neurosphere and the muscle strip.
  • movie S4 (.avi format). Muscle bundle contraction upon light simulation of the ChR-HBG3-MN in the microfluidic device.

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