Science Advances

This PDF file includes:

• Supplementary Materials and Methods
• fig. S1. DNA construct design, ESC colony morphology, DNA integration validation, and pluripotency characterization.
• fig. S2. Electrophysiological characterization of the ChR-HBG3-MN.
• fig. S3. Optogenetic activation of NMJs in adherent cocultures.
• fig. S4. Modes of tissue ablation.
• fig. S5. Photosensitization of the C2C12 myoblasts.
• fig. S6. Influence of the hydrogel on the force measured by the pillar.
• fig. S7. Motor neurite outgrowth in the microfluidic device.
• fig. S8. Opposite gradients as a mean to provide cell-specific medium.
• Legends for movies S1 to S4

Download PDF

Other Supplementary Material for this manuscript includes the following:

• movie S1 (.avi format). Muscle contraction upon optical stimulation of ChR-HBG3-MN in adherent cultures.
• movie S2 (.avi format). ECM compaction and muscle bundle formation over the first 96 hours of the experiment.
• movie S3 (.wmv format). 3D neurite outgrowth in the gel region bridging the neurosphere and the muscle strip.
• movie S4 (.avi format). Muscle bundle contraction upon light simulation of the ChR-HBG3-MN in the microfluidic device.