Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Conserved domains and active sites identified in the deduced Snn1 protein.
  • fig. S2. An unrooted phylogenetic tree showing relationships between Snn1 and other plant wall–associated receptor kinase (WAK) proteins.
  • fig. S3. Deduced amino acid sequence alignment of mutants and informative lines.
  • fig. S4. Transcription analysis of the splice site mutant CSems-6141.
  • fig. S5. DNA blot analysis.
  • fig. S6. DNA alignment of Snn1 from chromosome 1B and its putative homoeoallele from chromosome 1D.
  • fig. S7. Phylogenetic tree of 24 genotypes based on deduced amino acid sequences of the Snn1 gene.
  • fig. S8. Transcription analysis of Snn1 in the durum wheat variety Lebsock.
  • fig. S9. TaMAPK3 transcription analysis after SnTox1 spray inoculation.
  • fig. S10. Y2H assays showing that Snn1 interacts with SnTox1 in a sequence-specific manner.
  • table S1. PCR-based molecular markers used to anchor the CS chromosome 1BS BAC contig to the genetic linkage map containing the Snn1 gene.
  • table S2. PCR-based molecular markers developed for the seven candidate genes.
  • table S3. Descriptions of induced mutations identified within the Snn1 gene-coding region.
  • table S4. Top five BLASTP hits in the NCBI nr database using the Snn1 deduced amino acid sequence as a query.
  • table S5. The 826 Triticum accessions and 123 Ae. speltoides accessions evaluated for the presence of the Snn1 DNA sequence and/or for reaction to SnTox1.
  • table S6. Primers used for sequencing of Snn1 from genomic DNA.
  • table S7. PCR primers used to amplify the Snn1 cDNA fragments for sequencing.
  • table S8. PCR primers used to amplify the Snn1 cDNA 5′ and 3′ ends.
  • table S9. PCR primers used for RQ-PCR analysis.
  • table S10. PCR primers used for Y2H analysis.

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