Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Mass spectrometry results.
  • fig. S2. Western blot analysis of TAP-tagged proteins.
  • fig. S3. Materials for in vitro RNA binding experiments.
  • fig. S4. Loss of Jhd2 does not affect processing or polyA-dependent termination in vitro.
  • fig. S5. RT-qPCR and Western blot analysis for Jhd2 mRNA and protein used in PMA1 studies.
  • fig. S6. RT-qPCR and Western blot analysis for Jhd2 mRNA and protein used in KlCYC1 studies.
  • fig. S7. Metagene plots of H3K4me3 levels.
  • Legends for tables S1 to S3
  • table S4. Plasmids used in this study.
  • table S5. Yeast strains used in this study.
  • table S6. Primers used in this study.

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Other Supplementary Material for this manuscript includes the following:

  • table S1 (Microsoft Excel format). Jhd2 bound peaks identified using SICER.
  • table S2 (Microsoft Excel format). Pcf11 bound peaks identified using SICER.
  • table S3 (Microsoft Excel format). List of genes with APA after Jhd2 deletion.

Files in this Data Supplement: