Science Advances

Supplementary Materials

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  • Experimental design
  • fig. S1. HSC proliferative index is increased on collagen substrates.
  • fig. S2. CFU colony counts of cultured HSCs compared to those from freshly isolated HSCs.
  • fig. S3. Morphology of cultured HSCs with blebbistatin added to the culture medium.
  • fig. S4. Actomyosin contractility alters HSC matrix engagement and lineage specification.
  • fig. S5. Myosin-mediated actin cytoskeletal tension alters HSC lineage specification.
  • fig. S6. Role of integrins α5β1 and ανβ3 in myeloid lineage specification.
  • fig. S7. Immunofluorescence of protein-coated substrates.
  • fig. S8. FACS isolation of LSK cell population.
  • fig. S9. CFU colony enumeration.
  • fig. S10. Flow cytometric analysis of the colony-forming cells harvested from the CFU assay.
  • fig. S11. Mechanical characterization of PA gels.
  • table S1. Elastic modulus of PA gels.
  • References (57–60)

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