Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • table S1. MT and SPB parameters.
  • table S2. Motor and cross-linker parameters.
  • table S3. Parameters varied in model optimization.
  • table S4. Parameters altered for cross-linker–only model.
  • table S5. Strains used in this study.
  • Legends for videos S1 to S6
  • fig. S1. Results of SPB diffusion measurements.
  • fig. S2. Individual simulations of the model with simulated deletions of single species from Fig. 3 (A and B).
  • fig. S3. Individual simulations of the model lacking stabilization of MT dynamics by motors and cross-linkers from Fig. 3A.
  • fig. S4. Individual simulations of the model with varying kinesin-5 unbinding load sensitivity from Fig. 3 (C and D).
  • fig. S5. Individual simulations of the model with varying kinesin-14 unbinding load sensitivity from Fig. 3 (C and D).
  • fig. S6. Individual simulations of the model with varying cross-linker unbinding load sensitivity from Fig. 3 (C and D).
  • fig. S7. Individual simulations of the model with varying length of the tether linking MT minus ends to SPBs from Fig. 3 (E and F).
  • fig. S8. Individual simulations of the model with altered kinesin-5 direction reversal from Fig. 3 (G and H).
  • fig. S9. Individual simulations of the model with varying kinesin-14 and crosslinker numbers from Fig. 5B.
  • fig. S10. Individual simulations of the model with varying kinesin-5 and kinesin-14 numbers from Fig. 5C.

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Other Supplementary Material for this manuscript includes the following:

  • video S1 (.mov format). Simulation of the full model with reference parameter set.
  • video S2 (.mov format). Simulation of the model with short tether connecting MT minus ends to SPBs.
  • video S3 (.mov format). Simulation of the model with altered kinesin-5 bidirectionality.
  • video S4 (.mov format). Simulation of the model with a low kinesin-14 number and a low cross-linker number.
  • video S5 (.mov format). Simulation of the model with a low kinesin-5 number and a high kinesin-14 number.
  • video S6 (.mov format). Simulation of the model with a high kinesin-5 number and a low kinesin-14 number.

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