Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Light intensity, temperature, and pH over the 17 days, and DO saturation and depth for the first 14 days of the time series.
  • fig. S2. Temperature, pH, and DO saturation during the 1 hour before transcriptome sampling for each day of the 17-day experiment.
  • fig. S3. The magnitude of day-to-night variability in temperature, pH, and DO saturation for the time series.
  • fig. S4. Expression profiles in A. hyacinthus of the three environmentally responsive modules during the time series.
  • fig. S5. Temperature profiles during the time series for each colony of A. hyacinthus.
  • fig. S6. Results from the PCA of the 177 contigs in the UPR and calcium homeostasis module for each colony of A. hyacinthus.
  • fig. S7. Minimum, mean, and maximum temperature and pH for each time point in a day.
  • fig. S8. Trait heat map of the correlations between module eigengene values and the environmental variables.
  • Legends for tables S1 to S3
  • table S4. The 37 unique functional enrichment genes for the UPR and calcium homeostasis module in A. hyacinthus.
  • Legend for tables S5
  • table S6. The 34 unique functional enrichment genes for module RJ6 in A. hyacinthus.
  • table S7. The number of contigs in modules RJ6, RJ9, and RJ11 that are present in each of the gene modules identified by Rose and colleagues (18).

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Other Supplementary Material for this manuscript includes the following:

  • table S1( Microsoft Excel format). Contigs in each of the 13 modules for A. hyacinthus and their module membership.
  • table S2 (Microsoft Excel format). Results from ANOVA tests looking at the relationship of module expression in A. hyacinthus to multiple factors.
  • table S3 (Microsoft Excel format). Functional enrichment results for the UPR and calcium homeostasis module in A. hyacinthus.
  • table S5 (Microsoft Excel format). Functional enrichment results for module RJ6 in A. hyacinthus.

Files in this Data Supplement: