Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Exploded view of the simple construction.
  • fig. S2. Vacuum charging and long-term storage.
  • fig. S3. Detailed steps of micropatterning.
  • fig. S4. Digital plasma separation mechanism overview.
  • fig. S5. Failure of blood separation without the microcliff.
  • fig. S6. Optical signal obstructed when blood cells are not removed.
  • fig. S7. Microcliff gap effect on spatial robustness.
  • fig. S8. Reliable compartmentalization with smaller microcliff gap designs.
  • fig. S9. Selective particle separation according to size.
  • fig. S10. Total plasma volume separated versus time.
  • fig. S11. Microwell filling speed versus vacuum strength.
  • fig. S12. Vacuum battery system versus conventional degas pumping.
  • fig. S13. Vacuum lungs enable flow tuning.
  • fig. S14. Compartmentalization of all 224 microwells can be done in 12 min.
  • fig. S15. Consistent loading with the vacuum battery system.
  • fig. S16. RPA is more robust against plasma samples than LAMP and PCR.
  • fig. S17. Isothermal heating using reusable instant heat packs.
  • fig. S18. On-chip digital quantitative detection of MRSA DNA spiked in water.
  • note S1. Simulation of particle trajectories.
  • note S2. Diffusion through vacuum battery.
  • table S1. Primer, probe, and target sequences.
  • References (53–56)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1(.mov format). User protocol.
  • movie S2 (.mov format). Cross section.
  • movie S3 (.mov format). Digital plasma separation.
  • movie S4 (.mov format). Vacuum battery system.

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