Science Advances
Supplementary Materials
This PDF file includes:
- fig. S1. Time-course study of VirE2 subcellular localizations inside N. benthamiana cells.
- fig. S2. Accumulation of Agrobacterium at the intercellular space of infiltrated leaf epidermal cells.
- fig. S3. Accumulation of Agrobacterium-delivered VirE2 at the host plasma membrane.
- fig. S4. Colocalization of VirE2 with FM4-64–labeled endomembrane compartments in
N. benthamiana epidermal cells.
- fig. S5. Expression of Hub impaired FM4-64 uptake in N. benthamiana epidermal cells.
- fig. S6. Expression of dominant-negative clathrin Hub impaired VirE2 departure from
the plasma membrane in N. benthamiana epidermal cells.
- fig. S7. Agrobacterium-delivered VirE2 was associated with early endosomes rather than late endosomes.
- fig. S8. Mutations at other putative VirE2 endocytic sorting motifs did not affect
VirE2 internalization in host cells.
- fig. S9. Sequence alignment analysis of VirE2 from different types of Ti plasmids.
- table S1. Strains and plasmids used in the studies.
- Legends for movies S1 and S2
- References (69–71)
Other Supplementary Material for this manuscript includes the following:
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