Science Advances
Supplementary Materials
This PDF file includes:
- fig. S1. Absolute bacterial abundance per bee gut, as measured by 16S rRNA gene copies.
- fig. S2. Gut community similarities, compared using PCoA of binary Sørensen-Dice
dissimilarities at OTU97 clustering.
- fig. S3. NMDS plots of gut community Sørensen-Dice dissimilarities at OTU97 clustering.
- fig. S4. UPGMA clustering of communities based on Sørensen-Dice and unweighted
UniFrac distances.
- fig. S5. Linear mixed models testing of the effects of sampling location and host
identity on microbiome composition.
- fig. S6. Ancestral microbiome reconstructions using parsimony for the major
corbiculate bacterial phylotypes.
- fig. S7. Bayesian phylogeny of Lactobacillus Firm-5 based on the tuf gene.
- fig. S8. Maximum likelihood phylogeny of Lactobacillus Firm-5 based on the tuf gene.
- fig. S9. Phylogeny of Lactobacillus Firm-5 based on rpoA, and geographic associations of strains.
- fig. S10. Tanglegram of host bee and Lactobacillus Firm-5 phylogenetic topologies.
- fig. S11. Gut microbial diversity, as measured by Shannon's H on OTUs clustered at 99.5% similarity.
- fig. S12. Relationships between bee body size, gut community size (16S copies),
and microbiome diversity.
- fig. S13. Linear models testing of the effects of bee body size, gut community size
(16S copies), host phylogenetic affiliation (tribe), and bee colony size on microbiome
diversity.
- table S1. Bee size and colony size estimation and literature sources.
- table S2. Snodgrassella co-inoculation trials count data.
- Legends for data files S1 to S5
- References (122–148)
Other Supplementary Material for this manuscript includes the following:
- data file S1. List of samples and associated metadata used in this study.
- data file S2. OTU97 tables and curated taxonomic assignments.
- data file S3. OTU99.5 tables summarized by bacterial taxa, from samples rarefied to 5000 reads.
- data file S4. List of sequences deposited in GenBank.
- data file S5. Representative sequences from OTU97 and OTU99.5 clustering.
Files in this Data Supplement: