Science Advances

This PDF file includes:

• fig. S1. Absolute bacterial abundance per bee gut, as measured by 16S rRNA gene copies.
  
• fig. S2. Gut community similarities, compared using PCoA of binary Sørensen-Dice dissimilarities at OTU₉₇ clustering.
  
• fig. S3. NMDS plots of gut community Sørensen-Dice dissimilarities at OTU₉₇ clustering.
  
• fig. S4. UPGMA clustering of communities based on Sørensen-Dice and unweighted UniFrac distances.
  
• fig. S5. Linear mixed models testing of the effects of sampling location and host identity on microbiome composition.
  
• fig. S6. Ancestral microbiome reconstructions using parsimony for the major corbiculate bacterial phylotypes.
  
• fig. S7. Bayesian phylogeny of Lactobacillus Firm-5 based on the tuf gene.
  
• fig. S8. Maximum likelihood phylogeny of Lactobacillus Firm-5 based on the tuf gene.
  
• fig. S9. Phylogeny of Lactobacillus Firm-5 based on rpoA, and geographic associations of strains.
  
• fig. S10. Tanglegram of host bee and Lactobacillus Firm-5 phylogenetic topologies.
  
• fig. S11. Gut microbial diversity, as measured by Shannon's H on OTUs clustered at 99.5% similarity.
  
• fig. S12. Relationships between bee body size, gut community size (16S copies), and microbiome diversity.
  
• fig. S13. Linear models testing of the effects of bee body size, gut community size (16S copies), host phylogenetic affiliation (tribe), and bee colony size on microbiome diversity.
  
• table S1. Bee size and colony size estimation and literature sources.
  
• table S2. Snodgrassella co-inoculation trials count data.
  
• Legends for data files S1 to S5
  
• References (122–148)

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Other Supplementary Material for this manuscript includes the following:

• data file S1. List of samples and associated metadata used in this study.
  
• data file S2. OTU₉₇ tables and curated taxonomic assignments.
  
• data file S3. OTU_99.5 tables summarized by bacterial taxa, from samples rarefied to 5000 reads.
  
• data file S4. List of sequences deposited in GenBank.
  
• data file S5. Representative sequences from OTU₉₇ and OTU_99.5 clustering.