Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Discussion
  • fig. S1. Localization of APX to the intestinal cytoplasm of C. elegans.
  • fig. S2. Use of triplex reductive dimethylated samples to quantitatively compare GFP-APX-NES, GFP-APX-NLS, and GFP-only samples.
  • fig. S3. Reproducibility of the number of proteins identified from each location.
  • fig. S4. Identification of cytoplasm- or nucleus-localized proteins.
  • fig. S5. Effect of enrichment threshold on the identification of cytoplasm- or nucleus-localized proteins.
  • fig. S6. Quantitative mass spectrometry ratios of proteins selected for validation.
  • table S1. Comparison of existing techniques to determine protein localization in animals.
  • table S2. List of strains used in this study.
  • table S3. Pearson correlation coefficients of replicate samples.
  • table S5. Number of proteins measured compared to mRNA expressed in each tissue.
  • table S6. Total spectral counts for all identified proteins in each sample and NLS/NES scaling factors.

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Other Supplementary Material for this manuscript includes the following:

  • table S4 (Microsoft Excel format). Mass spectrometry data of spatially restricted enzymatic tagging in C. elegans.

Files in this Data Supplement: