Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Purification of recombinant human ATM.
  • fig. S2. Recombinant human ATM is catalytically active.
  • fig. S3. Cryo-EM images and 2D classes.
  • fig. S4. Reference models and 3D classes of human ATM.
  • fig. S5. Summary of the cryo-EM 3D classification and focused refinement of ATM structures.
  • fig. S6. FSC curves for the observed reconstructions.
  • fig. S7. Euler angle distribution of particles for each 3D class (A to E).
  • fig. S8. FSC between the ATM models and EM density maps.
  • fig. S9. Quality of the density for the highest-resolution human ATM map.
  • fig. S10. Comparison of the ATM FATKIN with mTOR.
  • fig. S11. Multiple sequence alignment of the FATKIN regions of human ATM and its representative orthologs, along with that of human mTOR.
  • fig. S12. Open and closed conformations of the ATM active site.
  • fig. S13. Domains of the N-solenoid of ATM.
  • fig. S14. Density bridging the two turns of the Spiral.
  • fig. S15. Comparison of structurally conserved domains in the N-solenoids of ATM and TOR.
  • fig. S16. Domain organization of ATM compared with TOR.
  • fig. S17. Highest-resolution subclass (8.4 Å resolution) of the open multiconformer dimer (from fig. S5, 3D class D) has one ordered protomer associated with a weakly ordered second FATKIN.
  • table S1. Data collection and refinement statistics.
  • table S2. Statistics for refined atomic models.
  • Legend for movie S1
  • References (51–58)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.mov format). Open and closed conformations of ATM.

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