Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Development of compliant PDMS substrates.
  • fig. S2. Differentiation and proliferation are supported on compliant silicone substrates.
  • fig. S3. PDGFR-β expression from mesodermal differentiation in serum.
  • fig. S4. Immunofluorescence microscopy of mature EC markers after mesoderm stiffness priming.
  • fig. S5. Differentiation and proliferation are supported on compliant silicone substrates in serum-free conditions.
  • fig. S6. PDGFR-β expression from mesodermal differentiation in serum-free conditions.
  • fig. S7. Immunofluorescence microscopy of EC markers after chemically defined mesoderm stiffness priming.
  • fig. S8. Immunofluorescence microscopy of EC markers after chemically defined mesoderm stiffness priming.
  • table S1. Literature review of techniques to induce mesodermal specification from hiPSCs.
  • table S2. Antibodies used in this study.
  • References (41, 42)

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