Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • fig. S1. Chemical priming of resting neutrophils leads to increase in CD11b expression.
  • fig. S2. Chemical priming of resting neutrophils leads to decrease in CD62L expression.
  • fig. S3. Higher ROS production in activated neutrophils compared to resting neutrophils.
  • fig. S4. Average aspect ratio during mechanical priming of neutrophils.
  • fig. S5. Mechanically induced depolarization of a chemically primed neutrophil.
  • fig. S6. Mechanically induced depolarization of GM-CSF–primed neutrophils.
  • fig. S7. Mechanically induced depolarization of a chemically primed neutrophil at a lower laser power.
  • fig. S8. Mechanically induced priming and depolarization of resting neutrophils.
  • fig. S9. Viability tests using trypan blue and annexin V/propidium iodide.
  • fig. S10. Delayed transit of activated cells in MMM at a lower temperature of 24°C.
  • Legends for movies S1 to S7
  • Other materials S1 to S3. Consent forms and questionnaire for blood donors.
  • References (56–59)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.wmv format). Mechanical deformation causes priming of resting neutrophils.
  • movie S2 (.wmv format). Mechanically induced depolarization of PMN (fMLP-treated).
  • movie S3 (.wmv format). Mechanically induced depolarization of PMN (GM-CSF–treated).
  • movie S4 (.wmv format). Mechanically induced depolarization of PMN in OS without thermal effects.
  • movie S5 (.avi format). RvE1-induced recircularization of mechanically polarized PMN.
  • movie S6 (.wmv format). Confirmation of OS results with MMM.
  • movie S7 (.wmv format). Activation of some resting cells in MMM constrictions.

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