Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. caDNAno DNA origami design.
  • fig. S2. Schematic DNA origami staple layouts of single-color metafluorophores (6 to 132).
  • fig. S3. Linear dependence of intensity on the number of dyes per DNA origami (calibrated).
  • fig. S4. Intensity distributions for 6 to 132 dyes.
  • fig. S5. Excitation power variation.
  • fig. S6. Integration time variation.
  • fig. S7. Refocusing performance.
  • fig. S8. Photostability.
  • fig. S9. Schematic DNA origami staple layouts of self-quenching study.
  • fig. S10. FRET investigation dye patterning (random and column-wise).
  • fig. S11. Intensity barcode dye patterns.
  • fig. S12. Intensity distributions for 124 barcodes in one sample.
  • fig. S13. Exemplary fluorescent image of nucleic acid detection.
  • fig. S14. DNA detection calibration.
  • fig. S15. Triggered assembly formation gel assay.
  • table S1. DNA origami staple sequences.
  • table S2. M13mp18 scaffold sequence.
  • table S3. Fluorescently labeled DNA sequences.
  • table S4. Intensity barcode subset (25 of 124).
  • table S5. Intensity barcode subset (12 of 64).
  • table S6. Intensity barcode subset (5 of 20).
  • table S7. DNA detection sequences and corresponding barcodes.
  • table S8. Triggered assembly sequences.
  • protocol S1. DNA origami self-assembly.
  • protocol S2. Microscopy sample preparation.
  • protocol S3. Triggered assembly on surface.
  • protocol S4. Triggered assembly in solution and gel assay.
  • Materials
  • Optical setup
  • Software section

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