Science Advances

This PDF file includes:

• fig. S1. Association of CD4 and CCR5 with lipid rafts in CD4⁺/CCR5⁺ HeLa cells with stably expressed CD4 and CCR5.
  
• fig. S2. Formation of GPMVs by treatment of CD4⁺/CCR5⁺ HeLa cells with small amounts of formaldehyde and DTT.
  
• fig. S3. Partitioning of CD4 and CCR5 in GPMVs induced by NEM instead of formaldehyde and DTT from CD4⁺/CCR5⁺ cells.
  
• fig. S4. HIV Env pseudovirus particles bind preferentially at boundaries between coexisting Lo and Ld domains in GPMVs.
  
• fig. S5. VSV-G pseudovirus particles bind to Ld membrane regions in GPMVs.
  
• fig. S6. Electron cryo-micrographs of HIV Env particles bound/fused to GPMVs.
  
• fig. S7. Modulation of lipid phases does not affect binding of HIV to GPMVs.
  
• fig. S8. Preparation of SPPMs with GPMVs.
  
• fig. S9. Lateral distribution of CD4/CCR5 in SPPMs.
  
• Legends for movies S1 to S9

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Other Supplementary Material for this manuscript includes the following:

• movie S1 (.avi format). Growing GPMVs on cell surfaces.
  
• movie S2 (.avi format). Coexisting fluid phases of cell-attached GPMVs.
  
• movie S3 (.avi format). HIV binding to cell-attached GPMVs.
  
• movie S4 (.avi format). HIV binding to the Lo/Ld boundaries in cell-attached blebs.
  
• movie S5 (.avi format). HIV binding to the Lo/Ld boundaries in cell-detached GPMVs.
  
• movie S6 (.avi format). Influence of MβCD on lipid phases of GPMVs.
  
• movie S7 (.avi format). Influence of lysoSM on lipid phases of GPMVs.
  
• movie S8 (.avi format). Influence of lysoSM on lipid phases of GUVs.
  
• movie S9 (.avi format). Fusion of HIV Env particles at Lo/Ld boundaries in an SPPM.

Download Movies S1 to S9