Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Association of CD4 and CCR5 with lipid rafts in CD4+/CCR5+ HeLa cells with stably expressed CD4 and CCR5.
  • fig. S2. Formation of GPMVs by treatment of CD4+/CCR5+ HeLa cells with small amounts of formaldehyde and DTT.
  • fig. S3. Partitioning of CD4 and CCR5 in GPMVs induced by NEM instead of formaldehyde and DTT from CD4+/CCR5+ cells.
  • fig. S4. HIV Env pseudovirus particles bind preferentially at boundaries between coexisting Lo and Ld domains in GPMVs.
  • fig. S5. VSV-G pseudovirus particles bind to Ld membrane regions in GPMVs.
  • fig. S6. Electron cryo-micrographs of HIV Env particles bound/fused to GPMVs.
  • fig. S7. Modulation of lipid phases does not affect binding of HIV to GPMVs.
  • fig. S8. Preparation of SPPMs with GPMVs.
  • fig. S9. Lateral distribution of CD4/CCR5 in SPPMs.
  • Legends for movies S1 to S9

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi format). Growing GPMVs on cell surfaces.
  • movie S2 (.avi format). Coexisting fluid phases of cell-attached GPMVs.
  • movie S3 (.avi format). HIV binding to cell-attached GPMVs.
  • movie S4 (.avi format). HIV binding to the Lo/Ld boundaries in cell-attached blebs.
  • movie S5 (.avi format). HIV binding to the Lo/Ld boundaries in cell-detached GPMVs.
  • movie S6 (.avi format). Influence of MβCD on lipid phases of GPMVs.
  • movie S7 (.avi format). Influence of lysoSM on lipid phases of GPMVs.
  • movie S8 (.avi format). Influence of lysoSM on lipid phases of GUVs.
  • movie S9 (.avi format). Fusion of HIV Env particles at Lo/Ld boundaries in an SPPM.

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