Science Advances
Supplementary Materials
This PDF file includes:
- fig. S1. Association of CD4 and CCR5 with lipid rafts in CD4+/CCR5+ HeLa cells with stably expressed CD4 and CCR5.
- fig. S2. Formation of GPMVs by treatment of CD4+/CCR5+ HeLa cells with small amounts of formaldehyde and DTT.
- fig. S3. Partitioning of CD4 and CCR5 in GPMVs induced by NEM instead of formaldehyde
and DTT from CD4+/CCR5+ cells.
- fig. S4. HIV Env pseudovirus particles bind preferentially at boundaries between
coexisting Lo and Ld domains in GPMVs.
- fig. S5. VSV-G pseudovirus particles bind to Ld membrane regions in GPMVs.
- fig. S6. Electron cryo-micrographs of HIV Env particles bound/fused to GPMVs.
- fig. S7. Modulation of lipid phases does not affect binding of HIV to GPMVs.
- fig. S8. Preparation of SPPMs with GPMVs.
- fig. S9. Lateral distribution of CD4/CCR5 in SPPMs.
- Legends for movies S1 to S9
Other Supplementary Material for this manuscript includes the following:
- movie S1 (.avi format). Growing GPMVs on cell surfaces.
- movie S2 (.avi format). Coexisting fluid phases of cell-attached GPMVs.
- movie S3 (.avi format). HIV binding to cell-attached GPMVs.
- movie S4 (.avi format). HIV binding to the Lo/Ld boundaries in cell-attached blebs.
- movie S5 (.avi format). HIV binding to the Lo/Ld boundaries in cell-detached GPMVs.
- movie S6 (.avi format). Influence of MβCD on lipid phases of GPMVs.
- movie S7 (.avi format). Influence of lysoSM on lipid phases of GPMVs.
- movie S8 (.avi format). Influence of lysoSM on lipid phases of GUVs.
- movie S9 (.avi format). Fusion of HIV Env particles at Lo/Ld boundaries in an SPPM.
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