Science Advances

Supplementary Materials

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  • fig. S1. PTM analyses of native Pif80.
  • fig. S2. Turbidimetric measurement of Ca2+-induced coacervation of rPif80 in the presence of 4 mM CaCl2.
  • fig. S3. SDS-PAGE analysis with Stains-All staining of rPif80.
  • fig. S4. Turbidimetric measurement of Ca2+-rPif80 coacervates according to additional NaCl.
  • fig. S5. Optical micrograph images (top) and Raman spectra (bottom) of mineralized CaCO3.
  • fig. S6. Cryo-scanning TEM image and EDS mapping analyses of rPif80-CLP.
  • fig. S7. Dot blotting with Coomassie staining after CaCO3-binding analysis of rPif80.
  • fig. S8. Structural analyses of a cross-sectioned plate mineral induced by rPif80 at a concentration of 50 μg/ml.
  • fig. S9. Morphology and polymorph analyses of grown minerals in the presence of protein impurities.

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