Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Comparison of effects of Sce, Pc, and Ph depletion on total RNA accumulation in BG3 cells.
  • fig. S2. Effects of Sce and Pc depletion on Ph occupancy of PREs, active promoters, and extragenic enhancers measured by ChIP-seq.
  • fig. S3. Effects of Sce and Ph depletion on Pc occupancy of PREs, active promoters, and extragenic enhancers measured by ChIP-seq.
  • fig. S4. PRC1 modifies Rpb1 phosphorylation at active genes.
  • fig. S5. Effects of Sce, Ph, and Pc depletion on Rpb3, Ser5P, Ser2P, and Spt5 occupancy of active promoters measured by ChIP-seq.
  • fig. S6. Effects of Sce and Ph depletion on Rpb3, Ser5P Rpb1, and Spt5 levels at extragenic enhancers and extragenic PREs measured by ChIP-seq.

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Other Supplementary Material for this manuscript includes the following:

  • table S1 (Microsoft Excel format). RNA-seq quantification and gene ontology.
  • table S2 (Microsoft Excel format). Statistical analysis of ChIP-seq data.
  • table S3 (Microsoft Excel format). Ribosomal RNA depletion oligonucleotides.
  • table S4 (Microsoft Excel format). ChIP-seq replicates and inputs used for normalization.
  • file S1 (.txt format). R scripts.
  • file S2 (.txt format). Whole-gene bed file for 3′ NT-seq quantification.
  • file S3 (.txt format). Active gene exons greater than 500 nt in length.
  • file S4 (.txt format). Potential active gene poly(A) signals.
  • file S5 (.txt format). Extended length active gene bed file for genes with high Ph and low H3K27me3 for metagene analysis.
  • file S6 (.txt format). Extended length active gene bed file for genes with low Ph and low H3K27me3 for metagene analysis.
  • file S7 (.txt format). Extended length active gene bed file for genes with high Ph and high H3K27me3 for metagene analysis.
  • file S8 (.txt format). Extended length active gene bed file for PcG domain genes for metagene analysis.

Files in this Data Supplement: