Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. EM of empty and substrate-bound ClpB in the presence of ATP-γ-S.
  • fig. S2. AMPPNP does not induce the substrate-binding ATP state of ClpB and Hsp104.
  • fig. S3. MD interactions.
  • fig. S4. ClpB pore loop mutants are affected in substrate binding and threading.
  • fig. S5. Focused 3D classification of the NTDs.
  • fig. S6. Refinement of the L2 tyrosine pore loop region of AAA2 of protomer F in the ground state.
  • fig. S7. Tabulation of the steps in data processing for ClpB and ClpB-casein data sets.
  • fig. S8. Details of the model fitted into the cryo-EM maps.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • movie S1(.mp4 format). Conformational changes of ClpB upon substrate binding.
  • movie S2 (.mov format). Corkscrew motion of the ClpB AAA2 hexamer to engage with the substrate.
  • movie S3 (.mov format). Pore loop motion upon substrate binding.

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