Science Advances

Supplementary Materials

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  • fig. S1. Expression of the daf-21p::mCherry reporter in the progeny of animals treated with RNAi targeting different subunits of the DNA polymerase complex and its associated proteins.
  • fig. S2. Increased transgene expression in div-1 mutants.
  • fig. S3. Maternal div-1 deficiency results in elevated transgene expression in the offspring.
  • fig. S4. Transgene up-regulation following pole-2 knockdown is suppressed in the mes-2;met-2;set-25 triple-mutant background.
  • fig. S5. Impaired DNA replication reduces H3K27me3 levels on multiple loci.
  • fig. S6. Global reduction of repressive histone marks and a gain of activating histone marks in late div-1(or148) embryos.
  • fig. S7. Knockdown of pole-2 results in reduction of H3K27me3 mark and increase in H3K4me3 in early embryonic chromatin.
  • fig. S8. Reduction in H3K9me3 mark in div-1(or148) mutant L1s detected by Western blot.
  • fig. S9. Passage of the transgenic array through impaired replication for a single generation is sufficient to trigger a multigenerational effect.
  • fig. S10. Quantification of H3K27me3 in interphase nuclei.
  • table S1. List of genes whose knockdown results in upregulation of daf-21p::mCherry transgene.
  • table S2. C. elegans strains used in this study.
  • table S3. Primers used in qPCR analyses.
  • table S4. Transgenes tested for derepression with div-1(RNAi).

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