Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Crystal structure of BbZIP.
  • fig. S2. Crystal packing of zinc-substituted BbZIP in two perpendicular directions.
  • fig. S3. Size exclusion chromatography of BbZIP in DDM.
  • fig. S4. Multiple sequence alignment of bacterial ZIPs.
  • fig. S5. A novel fold of BbZIP.
  • fig. S6. Tilted structure of BbZIP in the putative membrane.
  • fig. S7. Metal content analysis of the purified BbZIP by ICP-MS.
  • fig. S8. Structural comparison of M99.
  • fig. S9. Anomalous difference maps at M3 and M4 in the zinc-substituted structure.
  • fig. S10. Sequence alignment of BbZIP with hZIP4 TMD.
  • fig. S11. Comparison of hZIP4 structure models.
  • fig. S12. Determination of the zinc uptake kinetic parameters.
  • fig. S13. Proposed conformational change facilitated by metal release from the binuclear metal center.
  • fig. S14. The distances between the two metal-binding sites (M1 and M2) and S106.
  • fig. S15. Three independent zinc uptake assays.
  • fig. S16. Time-dependent zinc uptakes by HEK293T cells overexpressing hZIP4.
  • table S1. Crystallographic statistics.
  • table S2. Strains, plasmids, and primers.

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