Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Base editing efficiencies in UNG knockout cells.
  • fig. S2. CDA1-BE3 and AID-BE3 edit C’s following target G’s more efficiently than BE3.
  • fig. S3. Uneven editing in sites with multiple editable C’s results in lower product purity.
  • fig. S4. Base editing of multiple C’s results in higher base editing product purity.
  • fig. S5. Base editing of multiple C’s results in higher base editing product purity at the HEK3 and RNF2 loci.
  • fig. S6. BE4 induces lower indel frequencies than BE3, and Target-AID exhibits similar product purities as CDA1-BE3.
  • fig. S7. SaBE4 exhibits increased base editing yields and product purities compared to SaBE3.
  • table S1. Base editing outcomes from treatment with BE3, CDA1-BE3, AID-BE3, or APOBEC3G-BE3 at the EMX1 locus.
  • table S2. Base editing outcomes from treatment with BE3, CDA1-BE3, AID-BE3, or APOBEC3G-BE3 at the FANCF locus.
  • table S3. Base editing outcomes from treatment with BE3, CDA1-BE3, AID-BE3, or APOBEC3G-BE3 at the HEK2 locus.
  • table S4. Base editing outcomes from treatment with BE3, CDA1-BE3, AID-BE3, or APOBEC3G-BE3 at the HEK3 locus.
  • table S5. Base editing outcomes from treatment with BE3, CDA1-BE3, AID-BE3, or APOBEC3G-BE3 at the HEK4 locus.
  • table S6. Base editing outcomes from treatment with BE3, CDA1-BE3, AID-BE3, or APOBEC3G-BE3 at the RNF2 locus.
  • note S1. Python script to detect linkage disequilibrium in base editing outcomes at target sites with multiple target cytidines.
  • Supplementary Sequences. Amino acid sequences of CDA1-BE3, AID-BE3, BE3-Gam, SaBE3-Gam BE4, BE4-Gam, SaBE4, and SaBE4-Gam.

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