Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. The TIM23 complex and CL biogenesis.
  • fig. S2. The effect of CL synthase knockout on Tim50IMS functionality and
    association with the TIM23 complex.
  • fig. S3. Import and integration of 35STim23 into isolated mitochondria.
  • fig. S4. Response of 35STim23 cross-linking to decreased Δψm.
  • fig. S5. Incorporation of 35STim23 into nanodiscs: control tests.
  • fig. S6. Tim50IMS structural predictions and SAXS analyses.
  • fig. S7. SAXS analysis of Tim50CORE.
  • fig. S8. Effect of ionic strength on the Tim50IMS-bilayer interaction.
  • fig. S9. Quantitative analysis of Tim50IMS binding.
  • fig. S10. Structural features of the Tim50IMS homology model.
  • table S1. SAXS structural parameters.

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi format). Tim50IMS-bilayer no CL.
  • movie S2 (.avi format). Tim50IMS-bilayer with CL.

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