Science Advances

Supplementary Materials

This PDF file includes:

  • section S1. Static light scattering characterization
  • section S2. Contour of microgel images
  • section S3. Two-color superresolution microscopy—Calibration procedure
  • fig. S1. Form factor of pNIPAM microgels in dilute conditions.
  • fig. S2. Determination of microgel contours by using Fourier descriptors.
  • fig. S3. Assessment of correlations between size and shape.
  • fig. S4. Sample images of deformed microgels at 34 wt % with different values of IsoQ.
  • fig. S5. Vertices detected as maxima of curvature for deformed particles at different concentrations.
  • fig. S6. Distribution P(Lexp/Lth) at a concentration of 23.8 wt %.
  • fig. S7. Principle of two-color imaging by spectral demixing.
  • table S1. Average number of facets ‹n› as a function of concentration c in the final regime of isotropic compression.
  • table S2. SD of the distribution P(Lexp/Lth) characterizing the shape variation in the 2D images of microgels via the fluctuations of Lexp/Lth.

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.mp4 format). Animated 3D visualization of deformed microgels within a densely packed suspension at 25.6 wt %.

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