Science Advances

Supplementary Materials

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  • fig. S1. Optimizing the circle-sequencing assay.
  • fig. S2. The error rate of transcription is not affected by the expression level of a gene.
  • fig. S3. The error rate of transcription is not affected by the vicinity of a gene to an origin of replication.
  • fig. S4. The error rate of transcription is equal along the length of a gene.
  • fig. S5. Cell lines that display error-prone transcription do not exhibit elevated mutation frequencies.
  • fig. S6. Transcriptional deletion rate in WT and error-prone cell lines.
  • fig. S7. Multiple components of the purine synthesis and salvage pathways are affected in error-prone cells.
  • fig. S8. Multiple components of nitrogen metabolism are affected in error-prone cells.
  • fig. S9. Multiple components of NAD metabolism are affected in error-prone cells.
  • table S1. Distribution of synonymous, missense, and nonsense errors in WT and error-prone cell lines.
  • table S2. Genes significantly up-regulated >2-fold at the RNA level in rpb1E1103G cells.
  • table S3. Genes significantly up-regulated >2-fold at the RNA level in rpb9Δ cells.
  • table S4. Proteins significantly up-regulated >2-fold at the protein level in both rpb1E1103G and rpb9Δ cells.
  • References (42, 43)

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