Science Advances

Supplementary Materials

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  • fig. S1. Analysis of circular nucleosome array reconstitution by electrophoretic mobility shift assay.
  • fig. S2. Analysis of circular nucleosome array reconstitution by restriction enzyme digestion and electrophoretic DNA band-shift assay.
  • fig. S3. Binding of intercalator CQ to supercoiled DNA changes its electrophoretic mobility.
  • fig. S4. 2D gel electrophoresis of DNA extracted from NAs with 167- and 172-bp NRLs.
  • fig. S5. Modulating negative supercoiling in DNA minicircles in the presence of various concentrations of EtBr.
  • fig. S6. Preparation of supercoiled DNA constructs 167x12 and 172x12 relaxed by Topo I in the presence of EtBr (4.0 μg/ml).
  • fig. S7. Negative supercoiling of DNA minicircles with 207-bp NRL in the presence of EtBr.
  • fig. S8. Negative supercoiling of circular NAs with 207-bp NRL.
  • fig. S9. Linking number per nucleosome, ΔLk, in regular fibers with various linker lengths.

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