Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Gene expression during hPSC differentiation to BMECs.
  • fig. S2. BMECs differentiated from H9 hESCs and 19-9-11 iPSCs express EC- and BMEC-related proteins.
  • fig. S3. BMECs differentiated on Synthemax and vitronectin express EC- and BMEC-related proteins and have efflux transporter activities.
  • fig. S4. BMECs differentiated from hPSCs in defined and undefined protocols exhibit similar Pgp activities.
  • fig. S5. BMECs differentiated at different seeding densities express VEGFR2 and CD31.
  • fig. S6. BMECs differentiated at different seeding densities express Pgp.
  • fig. S7. BMECs differentiated at different densities express BMEC proteins but can exhibit aberrant protein localization.
  • fig. S8. TEER of BMECs differentiated from different hPSC lines.
  • fig. S9. TEER in BMECs differentiated from hPSCs at different seeding densities.
  • fig. S10. BMECs differentiated in the absence of RA exhibit low expression and mislocalization of EC and BMEC proteins.
  • fig. S11. RA enhances TEER for cells differentiated on Matrigel-, vitronectin-, and Synthemax-coated surfaces.
  • Legend for table S1
  • table S2. Antibodies used in this study.
  • table S3. qPCR primers used in this study.

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Other Supplementary Material for this manuscript includes the following:

  • table S1 (Microsoft Excel format). Expression of tight junction and transporter genes in iPSC-derived and primary human BMECs.

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