Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Gene coding region (CDS) inferred vertebrate opsin gene phylogeny.
  • fig. S2. Reconstruction of amino acid changes at known key spectral tuning sites of pearlside opsins.
  • fig. S3. Vertebrate Gα transducin gene phylogeny.
  • fig. S4. Vertebrate arrestin gene phylogeny.
  • fig. S5. rh2 opsin gene conversion phylogeny.
  • fig. S6. Topographic distribution of rod-like cone photoreceptors in three individuals of M. muelleri.
  • fig. S7. Topographic distribution of ganglion cells (excluding amacrine cells and glial cells) in three individuals of M. muelleri.
  • fig. S8. Expression of rh2 and rh1 opsin genes from sectional RNA in situ hybridization analyses of the eye of M. muelleri.
  • fig. S9. Location of the “true” rod photoreceptors and their distribution across the retina in M. muelleri.
  • fig. S10. Manual approach to extract genes from back-mapped reads.
  • table S1. Summary of transcriptomes, GenBank accession numbers, opsin mapping (including base pair coverage), and proportional opsin gene expression.
  • table S2. Summary of transcriptomes, GenBank accession numbers, transducin mapping (including base pair coverage), and proportional transducin gene expression.
  • table S3. Summary of transcriptomes, GenBank accession numbers, arrestin mapping (including base pair coverage), and proportional arrestin gene expression.
  • table S4. Single-gene GenBank accession numbers of gene sequences produced during this study.
  • table S5. Summary of the stereology parameters used for the analysis of the rod-like cone photoreceptors and ganglion cell distribution along with the quantitative results obtained using the optical fractionator methods in six retinas of M. muelleri.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.mp4 format). 3D reconstruction of the two photoreceptor types in M. muelleri.
  • movie S2 (.mp4 format). Close-up 3D reconstruction of the nucleus and synaptic terminal of the two photoreceptor types in M. muelleri.

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