Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Biosynthesis of wyosine derivatives in eukaryotic tRNAPhes.
  • fig. S2. The overall structure of the PaTrm5a-tRNAPhe-SAH ternary complex.
  • fig. S3. The asymmetric unit contents in the cocrystals of the PaTrm5a-tRNAPhe-SAH ternary complex.
  • fig. S4. The multiple sequence alignment of Trm5 sequences from different model organisms.
  • fig. S5. The interaction mode of U33/G34 in the PaTrm5a-tRNAPhe-SAH ternary complex.
  • fig. S6. Purity test of tRNA truncation mutants by size exclusion chromatography and Urea-PAGE.
  • table S1. Specific interactions between the enzyme and the tRNA substrate.
  • table S2. Statistics on SAXS data collection, analysis, and modeling.

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