Science Advances

This PDF file includes:

• fig. S1. Schematic of the fabrication procedure for nanowires anchored into PDMS.
  
• fig. S2. Nanowires anchored into PDMS.
  
• fig. S3. FESEM images and EDS elemental mappings corresponding to FESEM images for PDMS without nanowires, PDMS with buried nanowires, and nanowire-embedded PDMS; scale bars, 1 μm.
  
• fig. S4. Mechanical stability of anchored nanowires and nonanchored nanowires.
  
• fig. S5. Extraction process of miRNAs in urine using the nanowire-anchored microfluidic device, ultracentrifugation, and commercially available kits.
  
• fig. S6. FESEM images and EDS elemental mappings of an STEM image of a single nanowire.
  
• fig. S7. Detection of EVs on ZnO nanowires, ZnO/Al₂O₃ core-shell nanowires, and no nanowires using an antibody of CD9.
  
• fig. S8. Zeta potential of EVs in urine.
  
• fig. S9. Size distribution of EVs collected by ultracentrifugation and EV-free miRNAs collected onto nanowires.
  
• fig. S10. Size distribution of the urinary free-floating objects.
  
• Legend for movie S1
  
• Legend for data S1

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Other Supplementary Material for this manuscript includes the following:

• movie S1 (.mov format). EV collection followed by miRNA extraction in urine using the nanowire-anchored microfluidic device.
  
• data S1 (Microsoft Excel format). Logarithmic signal intensities in noncancer miRNAs with those in cancer miRNAs.