Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Methods
  • Legends for movies S1 to S5
  • fig. S1. Steady-state actin-activated ATPase rates and ATP-induced dissociation of actomyosin.
  • fig. S2. Contour plots showing R2 dependence of fit of Eq. 5 to data from Fig. 3B.
  • fig. S3. Contour plots showing R2 dependence of fit of Eq. 5 to data from Fig. 2 (A to C).
  • fig. S4. Distance from origin plot of actin filament moving over a myosin filament in the A/Mf assay.
  • fig. S5. Effect of rhodamine labeling and EDC cross-linking on CMM
    steady-state actin-activated ATPase activity.
  • fig. S6. Flowchart describing algorithm to solve recursive Eq. 5.
  • Supplementary Text
  • Reference (59)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.mp4 format). Cartoon depicting detachment-limited kinetics in the A/Mm assay.
  • movie S2 (.mp4 format). Cartoon depicting mixed kinetics (attachment to detachment) in the Mf/A assay.
  • movie S3 (.avi format). Example movie of SKM filaments moving in the Mf/A assay.
  • movie S4 (.avi format). Example movie of CMM filaments moving in the Mf/A assay.
  • movie S5 (.avi format). Example movie of actin filaments moving over SKM filaments in the A/Mf assay.

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