Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Representative full-spectrum MRI series of livers from PBS-, vector-, and mTSG-treated mice.
  • fig. S2. Additional bright-field images of mTSG-treated livers with GFP overlay.
  • fig. S3. Representative full-slide scanning images of mouse liver sections in PBS, vector, and mTSG treatment groups.
  • fig. S4. Representative histology and immunohistochemistry images of mouse liver sections in PBS, vector, and mTSG groups.
  • fig. S5. MIP capture sequencing statistics and indel size distribution of mTSG livers.
  • fig. S6. Mutated sgRNA sites across all liver samples from mice treated with AAV-mTSG library.
  • fig. S7. Heat map of gene-level sum variant frequency across all mTSG liver samples.
  • fig. S8. Additional co-mutation analysis.
  • fig. S9. Heat map of all unique variants across all mTSG liver samples.
  • fig. S10. Investigation and comparison of single or combinatorial knockout of screened TSGs in liver tumorigenesis.
  • fig. S11. Mutant clonality and clustering analysis.
  • Legends for tables S1 to S17

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • table S1 (Microsoft Excel format). DNA sequences of sgRNA spacers in mTSG library.
  • table S2 (Microsoft Excel format). Raw read counts of mTSG plasmid library.
  • table S3 (Microsoft Excel format). Tumor volume data as measured by MRI.
  • table S4 (Microsoft Excel format). Survival data for PBS-, vector-, or
    mTSG-treated animals.
  • table S5 (Microsoft Excel format). Tumor area data as measured by tissue histology.
  • table S6 (Microsoft Excel format). Sequence information and annotation for all MIPs used in the study.
  • table S7 (Microsoft Excel format). Metadata for all of the 133 sequenced samples.
  • table S8 (Microsoft Excel format). MIP capture sequencing coverage statistics across all predicted cutting sites of sgRNAs in AAV-mTSG library.
  • table S9 (Microsoft Excel format). Raw indel variant calls of all samples with targeted capture sequencing before filtering.
  • table S10 (Microsoft Excel format). sgRNA-level sum indel frequency table for all samples with targeted capture sequencing.
  • table S11 (Microsoft Excel format). sgRNA-level binary MS calls in livers from mice treated with AAV-mTSG library.
  • table S12 (Microsoft Excel format). Gene-level binary MG calls in livers from mice treated with AAV-mTSG library.
  • table S13 (Microsoft Excel format). Co-occurrence analysis of MG pairs in livers from mice treated with AAV-mTSG library.
  • table S14 (Microsoft Excel format). Correlation analysis of gene-level sum indel frequency in livers from mice treated with AAV-mTSG library.
  • table S15 (Microsoft Excel format). Mutant frequencies for all unique variants present across all mTSG liver samples.
  • table S16 (Microsoft Excel format). Spearman rank correlation matrix for five individual liver lobes within a single mouse.
  • table S17 (Microsoft Excel format). Mutant frequencies for all unique variants present in five individual liver lobes from a single mouse.

Download Tables S1 to S17

Files in this Data Supplement: