Science Advances

Supplementary Materials

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  • Expression and purification of the recombinant A2 domain
  • fig. S1. Differential cysteine alkylation and mass spectrometry analysis of the VWF Cys1669-Cys1670 disulfide bond.
  • fig. S2. FDA of the VWF A2 domain.
  • fig. S3. Concordance between RMSF for the simulated A2 redox states and crystallographic B factors.
  • fig. S4. Anticoagulation of blood with calcium chelators (citrate or EDTA) or direct thrombin inhibitors (heparin or hirudin) does not appreciably influence the redox state of the A2 domain cysteines.
  • fig. S5. Plasma VWF collected on antibody-coated beads, resolved on reducing SDS-PAGE, and stained with colloidal Coomassie.

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