Science Advances

Supplementary Materials

This PDF file includes:

  • section S1. Endosome position determination
  • section S2. Rotation of detached endosome
  • section S3. Estimation of endosome size and hydrodynamic drag during transport
  • section S4. Reduction in nanorod-microtubule alignment after disruption of the ligand-receptor interaction
  • fig. S1. System precision calibration.
  • fig. S2. Estimation of minimal imaging speed from freely tumbling endosome.
  • fig. S3. Different pauses of the same endosome can be rotationally distinct.
  • fig. S4. Angular velocity correlates with motion of the endosome perpendicular to the microtubule.
  • fig. S5. Endosome rotational motion is not a function of local axonal environment.
  • fig. S6. Reversals have statistically significant increase in rotation.
  • fig. S7. Alignment distribution in individual experiments.
  • fig. S8. Median endosome orientation is still aligned with microtubules.
  • fig. S9. Anterograde endosome alignment not due to hydrodynamic forces.
  • fig. S10. Rotational tracking of freely diffusing endosome in the axon.
  • fig. S11. Orientational memory maintained for orientations perpendicular to the microtubule track.
  • fig. S12. Alignment of nanorods to microtubules after ligand-receptor interaction cleavage.
  • fig. S13. Sample nanorod-endosome-microtubule images.
  • Legends for movies S1 to S8
  • Reference (43)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi format). Corresponds to fig. S2.
  • movie S2 (.avi format). Corresponds to trace in Fig. 3D in the main text.
  • movie S3 (.avi format). Corresponds to bottom kymograph in Fig. 3C the main text.
  • movie S4 (.avi format). Retrograde-directed endosome from Fig. 5A showing correlations between angular velocity and translational acceleration.
  • movie S5 (.avi format). Corresponds to Fig. 6A in the main text.
  • movie S6 (.avi format). Corresponds to Fig. 7C in the main text.
  • movie S7 (.avi format). Corresponds to Fig. 7A in the main text.
  • movie S8 (.avi format). Corresponds to Fig. 8C in the main text.

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