Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Locations of the human KCNQ1 mutation examined in this work.
  • fig. S2. Fluorimetric cell flow cytometry assay used to determine total and surface protein expression levels.
  • fig. S3. Surface trafficking efficiency for each mutant juxtaposed with KCNQ1 peak current density.
  • fig. S4. Effect of coexpression of KCNE1 with KCNQ1 on total surface expression levels of KCNQ1.
  • fig. S5. 1H/15N-TROSY NMR spectra (900 MHz) of KCNQ1 mutants (red) superimposed on the spectrum of WT KCNQ1 (black).
  • fig. S6. Results from MD trajectories.
  • fig. S7. Hydrogen bond interactions of the KCNQ1 VSD with solvent are observed during MD simulation.
  • fig. S8. Correlation plot of residue motions calculated from KCNQ1 VSD MD simulations.
  • table S1. Functional and trafficking results for 51 human KCNQ1 mutants.
  • table S2. List of nonbonded interactions of LQTS1 and VUS mutation sites in S0 and S0-contacting regions observed during MD simulation of the KCNQ1 VSD.

Download PDF

Files in this Data Supplement: