Science Advances

Supplementary Materials

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  • Supplementary Text
  • fig. S1. Characterization of S. cerevisiae PADH2-like promoters.
  • fig. S2. Cloning vectors used in this study.
  • fig. S3. Improving DNA assembly.
  • fig. S4. Schematics of all PKS-containing BGCs examined here.
  • fig. S5. UTC-containing BGCs examined here.
  • fig. S6. Volcano plot of all spectral features identified in the automated analysis
    of strains expressing PKS-containing BGCs.
  • fig. S8. All features produced by PKS1 in strain 132.
  • fig. S9. All features produced by PKS2 in strain 133.
  • fig. S10. All features produced by PKS4 in strain 255.
  • fig. S11. All features produced by PKS6 in strain 178.
  • fig. S12. All features produced by PKS8 in strain 164.
  • fig. S13. All features produced by PKS10 in strain 246.
  • fig. S14. All features produced by PKS13 in strain 206.
  • fig. S15. All features produced by PKS14 in strain 257.
  • fig. S16. All features produced by PKS15 in strain 247.
  • fig. S17. All features produced by PKS16 in strain 177.
  • fig. S18. All features produced by PKS17 in strain 176.
  • fig. S19. All features produced by PKS18 in strain 207.
  • fig. S20. All features produced by PKS20 in strain 208.
  • fig. S21. All features produced by PKS22 in strain 209.
  • fig. S22. All features produced by PKS23 in strain 241.
  • fig. S23. All features produced by PKS24 in strain 210.
  • fig. S24. All features produced by PKS28 in strain 240.
  • fig. S25. 1H NMR spectrum of compound 6 in CDCl3.
  • fig. S26. 13C NMR spectrum of compound 6 in CDCl3.
  • fig. S27. 1H-1H COSY spectrum of compound 6 in CDCl3.
  • fig. S28. HSQC spectrum of compound 6 in CDCl3.
  • fig. S29. HMBC spectrum of compound 6 in CDCl3.
  • fig. S30. 1H NMR spectrum of compound 7 in acetone-d6.
  • fig. S31. 13C NMR spectrum of compound 7 in acetone-d6.
  • fig. S32. 1H-1H COSY spectrum of compound 7 in acetone-d6.
  • fig. S33. HSQC spectrum of compound 7 in acetone-d6.
  • fig. S34. HMBC spectrum of compound 7 in acetone-d6.
  • fig. S35. 1H NMR spectrum of compound 8 in acetone-d6.
  • fig. S36. 13C NMR spectrum of compound 8 in acetone-d6.
  • fig. S37. 1H-1H COSY spectrum of compound 8 in acetone-d6.
  • fig. S38. HSQC spectrum of compound 8 in acetone-d6.
  • fig. S39. HMBC spectrum of compound 8 in acetone-d6.
  • fig. S40. 1H NMR spectrum of compound 9 in CDCl3.
  • fig. S41. 13C NMR spectrum of compound 9 in CDCl3.
  • fig. S42. 1H-1H COSY spectrum of compound 9 in CDCl3.
  • fig. S43. HSQC spectrum of compound 9 in CDCl3.
  • fig. S44. HMBC spectrum of compound 9 in CDCl3.
  • fig. S45. 1H NMR spectrum of compound 10 in CDCl3.
  • fig. S46. 13C NMR spectrum of compound 10 in CDCl3.
  • fig. S47. 1H-1H COSY spectrum of compound 10 in CDCl3.
  • fig. S48. HSQC spectrum of compound 10 in CDCl3.
  • fig. S49. HMBC spectrum of compound 10 in CDCl3.
  • fig. S50. 1H NMR spectrum of compound 11 in CDCl3.
  • fig. S51. 13C NMR spectrum of compound 11 in CDCl3.
  • fig. S52. 1H NMR spectrum of compound 12 in CDCl3.
  • fig. S53. 13C NMR spectrum of compound 12 in CDCl3.
  • fig. S54. 1H-1H COSY spectrum of compound 12 in CDCl3.
  • fig. S55. HSQC spectrum of compound 12 in CDCl3.
  • fig. S56. HMBC spectrum of compound 12 in CDCl3.
  • fig. S57. NOESY spectrum of compound 12 in CDCl3.
  • fig. S58. 1H NMR spectrum of compound 13 in CDCl3.
  • fig. S59. 13C NMR spectrum of compound 13 in CDCl3.
  • fig. S60. 1H-1H COSY spectrum of compound 13 in CDCl3.
  • fig. S61. HSQC spectrum of compound 13 in CDCl3.
  • fig. S62. HMBC spectrum of compound 13 in CDCl3.
  • fig. S63. NOESY spectrum of compound 13 in CDCl3.
  • fig. S64. 1H NMR spectrum of compound 14 in CDCl3.
  • fig. S65. 13C NMR spectrum of compound 14 in CDCl3.
  • fig. S66. 1H-1H COSY spectrum of compound 14 in CDCl3.
  • fig. S67. HSQC spectrum of compound 14 in CDCl3.
  • fig. S68. HMBC spectrum of compound 14 in CDCl3.
  • fig. S69. 1H NMR spectrum of compound 15 in CDCl3.
  • fig. S70. 13C NMR spectrum of compound 15 in CDCl3.
  • fig. S71. 1H-1H COSY spectrum of compound 15 in CDCl3.
  • fig. S72. HSQC spectrum of compound 15 in CDCl3.
  • fig. S73. HMBC spectrum of compound 15 in CDCl3.
  • fig. S74. HMBC spectrum of compound 15 in CDCl3.
  • fig. S75. 1H NMR spectrum of compound 16 in CDCl3.
  • fig. S76. 13C NMR spectrum of compound 16 in CDCl3.
  • fig. S77. 1H-1H COSY spectrum of compound 16 in CDCl3.
  • fig. S78. HSQC spectrum of compound 16 in CDCl3.
  • fig. S79. HMBC spectrum of compound 16 in CDCl3.
  • table S1. Ion source parameters used in this study.
  • table S2. Expression data for selected promoters drawn from genome-wide expression studies.
  • table S3. Sequences of HEx promoters.
  • table S4. Background strains used throughout this study.
  • table S5. Features integrated for the determination of sesquiterpenoid titer in Fig. 2B.
  • table S6. Order of promoters and terminators used in the expression of all cryptic fungal BGCs examined in this study.
  • table S7. Standard part plasmids and expression vectors used for the assembly of cryptic BGCS in this study.
  • table S8. Coordinates of native loci from which all clusters examined here were derived along with the IDs of plasmids expressing the engineered cluster versions.
  • table S9. Abbreviations for the functional gene annotations used in Figs. 2, 5, and 6.
  • table S10. Strains expressing cryptic fungal BGCs analyzed here.
  • table S11. NMR data of compound 6.
  • table S12. NMR data of compound 7.
  • table S13. NMR data of compound 8.
  • table S14. NMR data of compound 9.
  • table S15. NMR data of compound 10.
  • table S16. NMR data of compound 11.
  • table S17. NMR data of compound 12.
  • table S18. NMR data of compound 13.
  • table S19. NMR data of compound 14.
  • table S20. NMR data of compound 15.
  • table S21. NMR data of compound 16.
  • References (70–73)

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