Science Advances

Supplementary Materials

This PDF file includes:

  • section S1. Characterization
  • section S2. Synthesis and experimental details of bacterium-shaped hollow silica particles
  • section S3. Determining the anchoring point of a cell and classifying its dynamics
  • section S4. Adhesion for mutants without the flagellar hook
  • section S5. Pivoters, wobblers, and deserted adhesion sites
  • section S6. MSDs of anchoring point for wobblers and pivoters
  • section S7. Kinetic model details
  • fig. S1. Electron microscopy images of E. coli WT and ΔFF and bacterium-shaped hollow silica particles.
  • fig. S2. AFM images of the inner surface of the glass capillaries.
  • fig. S3. MSDs and anchoring points on the length axis of different types of bacteria.
  • fig. S4. Distributions of rotational exponents kR for mutants lacking essential hook proteins.
  • fig. S5. Pivoters, wobblers and deserted binding sites for E. coli WT and ΔFF.
  • fig. S6. MSDs and τ for pivoters and wobblers.
  • table S1. The cell numbers used for simulating the dynamics of bacterial adhesion.
  • Legends for movies S1 to S11
  • References (58–60)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1(.avi format). Determining positions and orientations of bacteria within the field of view.
  • movie S2 (.avi format). Lower surface before inversion for WT, categorized cells.
  • movie S3 (.avi format). Fifteen examples of trajectories for WT cells identified as diffusing.
  • movie S4 (.avi format). Fifteen examples of trajectories for WT cells identified as swimming.
  • movie S5 (.avi format). Fifteen examples of trajectories for WT cells identified as adhering and wobbling.
  • movie S6 (.avi format). Fifteen examples of trajectories for WT cells identified as adhering and pivoting.
  • movie S7 (.avi format). Fifteen examples of trajectories for WT cells identified as adhering and actively rotating.
  • movie S8 (.avi format). Examples of adhering cells with stained flagella (AD14) that are actively rotating.
  • movie S9 (.avi format). Illustration of deserted binding sites on the surface in time for ΔFF.
  • movie S10 (.avi format). Computer simulations of the inversion experiment for E. coli WT using a simple kinetic model.
  • movie S11 (.avi format). Computer simulations of the inversion experiment for ΔFF using a simple kinetic model.

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